Title: Effects of fructose sodium diphosphate on expression of CHOP and JNK in endoplasmic reticulum stress and islet apoptosis in rats with type 2 diabetes
Abstract: AIM: To study the effects of fructose sodium diphosphate(FDP) on the expression of CHOP and c-Jun N-terminal binase(JNK) in endoplasmic reticulum stress and islet apoptosis in the rats with type 2 diabetes mellitus(T2DM).METHODS: T2DM model was established in male Wistar rats by feeding of high lipid diet and injection of streptozotocin.The rats were divided into 4 groups(n=8):normal control group,T2DM model group,T2DM+low-dose FDP(2 mL·kg-1·d-1,ip) group and T2DM+high-dose FDP(5 mL·kg-1·d-1,ip) group.The rats in the treatment groups received FDP for 8 weeks.The levels of fasting blood glucose(FBG),fasting serum insulin(FINS) and insulin sensitivity index(ISI) were measured.TUNEL was used to detect the islet apoptosis.The protein levels of CHOP and JNK were determined by the method of immunohistochemistry.RESULTS: (1) Compared with normal control group,FBG,FINS,the expression of CHOP and JNK,and apoptosis in T2DM model group were significantly increased(P0.01).The level of ISI was significantly decreased.(2) Compared with T2DM model group,the levels of FBG and FINS,the expression of CHOP and JNK,and apoptosis in high-dose FDP group were significantly decreased.The level of ISI was significantly increased(P0.01).However,the level of FBG,the expression of CHOP and JNK,and apoptosis in low-dose FDP group were significantly decreased.Compared with low-dose FDP group,the levels of FBG and FINS,the expression of CHOP and JNK,and apoptosis in high-dose FDP group were significantly decreased.The level of ISI was significantly increased(P0.01 or P0.05).CONCLUSION: FDP may prevent islet cells from apoptosis in T2DM rats by decreasing the expression of CHOP and JNK.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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Cited By Count: 1
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