Title: Expression pattern and function of microRNA-518b in invasive ductal breast cancer
Abstract: Objective To explore the expression of miR-518b in invasive ductal breast cancer and analyze its impact on the proliferation,cell cycle,apoptosis and invasion of MCF-7 breast cancer cell line.Methods Twenty pairs of breast cancer and normal tissue specimens were chosen after confirmation of breast invasive ductal carcinoma.We conducted reverse transcription-polymerase chain reaction (RT-PCR) to quantify the expression of miR-518b,and its correlation with clinical data was analyzed by using SPSS software 13.0.To characterize the effect of miR-518b on MCF-7 breast cancer cells,functional assays were respectively employed,including proliferation,cell cycle,apoptosis,and Transwell assays.Results The relative expression of miR-518b in breast cancer specimens was 2.751 585 ±5.856 351,which was significantly lower than 7.334 841 ± 9.843 424 of adjacent normal ones ( P < 0.01 ).Aberrant expression of miR-518b was correlated with lymphatic metastasis (P < 0.O1 ).On the contrary,no correlation was observed of miR-518b with TNM stage,tumor size,HER-2,estrogen receptor (ER),progesterone receptor (PR) and p53 status.Methyl thiazol tetrazolium (MTT) assay revealed that miR-518b mimics significantly inhibited proliferation of breast tumor cells at 48 h after transfection at the concentration of 150 nmol/L with the inhibition rate being 59.9%.In comparison to controls,there was no significant difference in the percentage of G0/G1 phase cells ( 75.52 ± 1.78 ) %,S phase cells (9.52 ± 0.91 ) % and the proportion of early apoptotic cells (2.60 +0.06) % after miR-518b mimics transfection ( P >0.05).The amount of invaded breast cancer cells in miR-518b transfected groups was significantly different from control groups and declined with an increased miR-518b concentration.Conclusion miR-518b expression is aberrantly expressed in breast cancer.Breast tumor specimens show decreased miR-518b expression compared to their normal counterparts.Considering the inhibitory impact of miR-518b on cell proliferation and invasion,miR-518b might potentially act as tumor suppressor gene in breast cancer.
Key words:
Breast carcinoma; microRNA-518b; Proliferation; Cell cycle
Publication Year: 2012
Publication Date: 2012-05-08
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot