Title: Clinical significance of microRNA-199a expression in gastric cancer
Abstract: Objective To investigate the expression of miR-199a-3p and miR-199a-5p and its association with clinicopathologic features in gastric cancer.Methods The expression levels of miR-199a-3p and miR-199a-5p in 32 gastric cancers and their matched non-tumor adjacent tissue specimens were detected by using stem-loop real-time reverse transcription-polymerase chain reaction (RT-PCR),and their correlation with clinicopathologic features of gastric cancer was analyzed.Results Expression levels of miR199a-3p and miR-199a-5p were significantly higher in tumor tissues than in adjacent normal tissues ( P <0.05 ).The expression of miR-199a-3p was associated with lymph node metastasis positively (P < 0.01 ),TNM stage (P <0.05) and cell differentiation (P <0.01 ).The expressions of miR-199a-5p was associated with cell differentiation ( P < 0.01 ) and location ( P < 0.05 ).ROC curve of miR-199a-3p and miR199a-5p about cell differentiation resulted in a diagnostic sensitivity of 93.3% and spectificity of 71.4%.Conclusion The level of miR-199a-3p and miR-199a-5p expression may be a valuable adjuvant parameter in forecasting the poorly differentiated gastric cancer.
Key words:
microRNA; Gastric carcinoma; Cell differentiation
Publication Year: 2011
Publication Date: 2011-12-08
Language: en
Type: article
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