Title: Expression of miR-126 in human colon cancer cells and its effect on drug resistance
Abstract: Objective To investigate the expression of miR-126 in human colon cancer cells and its effect on drug resistance. Methods Forty five cases of colon cancer tissues, para-carcinoma tissues and 30 cases of normal colon tissues were collected from July 2010 to August 2013 in Department of Oncology of our hospital. By detecting miR-126 expression levels using fluorescent PCR, culturing HCT116 cells in vitro, suppressing the expression of miR-126 by transfecting the suppressing sequence, we studied the drug resistance effect of miR-126 in HCT116 cells. Results The positive rate of miR-126 expression in colon cancer tissues was significantly lower than that in para-carcinoma tissues and normal tissues(P 0.05); moreover, this rate in para-carcinoma tissues was also lower than that in normal tissues, but the difference was not significant(P 0.05). No significant differences of miR-126 expression in age, gender, differentiation,and, tumor size were observed(P 0.05), but miR-126 expression was found to be associated with lymph node metastasis, Dukes′staging and clinical staging; the differences among miR-126 expression levels was also significant(P 0.05).In 0, 5, 10,20 mg/L oxaliplatin medium, cancer cell activity(absorbance) in miR-126 positive group was significantly lower than in miR-126 negative group(P 0.05). Conclusion The positive expression of miR-126 in colon cancer tissues is relatively low, whereas this positive expression can reduce drug resistance of cancer cells and is beneficial to the treatment of colon cancer.
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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