Title: [The expression and significance of the angiopoietin-1 in the kidney of diabetic rats].
Abstract:To explore the expression and significance of angiopoietin-1 (Ang-1) in the renal tissue of diabetic rats.The SD rats were divided into the diabetic and control groups. The diabetic group was treated ...To explore the expression and significance of angiopoietin-1 (Ang-1) in the renal tissue of diabetic rats.The SD rats were divided into the diabetic and control groups. The diabetic group was treated by streptozotocin. The expression of Ang-1 in renal tissue was detected by RT-PCR and immunohisochemistry at 2, 4, 8, 12, 16, 20 and 24 weeks. The level of Ang-1 was quantified by computer image analysis. The relationship between Ang-1 and the index of renal damage including renal/body weight, urinary protein excretion, glomerular volume, and glomerular area was analyzed.Ang-1 mRNA in diabetic renal tissue was significantly upregulated at 4 and 8 weeks, compared with diabetic group at other time points or control group at the same time points, and then downregulated gradually after 12 weeks. The level of Ang-1 mRNA decreased significantly as compared with control group at 24 week. Ang-1 was outstandingly expressed in glomeruli. From 4-week to 24-week, the number of Ang-1 staining in diabetic glomeruli increased significantly as compared with control group, being maximal at 4 and 8 weeks, and subsequently decreased after 12 week. Ang-1 level was correlated with renal/body weight, glomerular volume, glomerular area, and urine protein excretion, respectively.The change of Ang-1, which is early upregulation and late downregulation, exists in diabetic renal tissue. The unusual expression of Ang-1 is partly connected with the renal changes of diabetic rats.Read More
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
Indexed In: ['pubmed']
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Cited By Count: 4
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