Title: The Role of MCP-1 in Hypertensive Renal Injury of NO-deficient Rat
Abstract:Objective To study the role of MCP-1 in hypertensive renal injury of NO-deficient Rat.Methods The model of NO-deficient hypertesive renal injury rat was made by giving heavy dose (N-Nitro-L-Argininem...Objective To study the role of MCP-1 in hypertensive renal injury of NO-deficient Rat.Methods The model of NO-deficient hypertesive renal injury rat was made by giving heavy dose (N-Nitro-L-Argininemethyl easter,L-NAME)(hypertension group,n=10).Rats were sacrificed after 8 weeks.The renal tissues pathologic change was investgated with light microscope.24 h urinary protein(Up) and β2-microglobucin(β2-m) exeretion were measured.The level of Cr was measured in plasma.The level of angiotensin Ⅱ(AngⅡ) were measured in plasma and renal tissue.The difference of monocyte chemoattractant protein-1(MCP-1) was compared with control group in renal tissue through western blotting.Results As compared to control group,Up and β2-m showed significantly increased at week 8 in hypertension group;Glomerulosclerosis and interstitial fibrosis index elevated apparently in renal tissue of hypertension group;AngⅡ(96.2±18.3 vs 45.3±14.6,P0.01) and MCP-1(3.68± 0.25 vs 1.76±0.34,P0.05)stayed significant high level in renal tissue of hypertension group.Conclusions MCP-1 plays an important role in the development of hypertensive renal injury induced by NO-deficient,and AngⅡ-MCP-1-ECM axis maybe a major pathological feature in the modle.Read More
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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