Title: A study of apoptosis of substantia nigra neurons in 6-hydroxydopamine-induced Parkinson's disease model rats
Abstract: Objective To investigate the possible death mode of dopaminergic neurons during the development of Parkinson's disease(PD). Methods TdT mediated biotinylated dUTP nick end labeling(TUNEL) method, flow cytometry and immunohistochemistry were performed for apoptotic detection of substantia nigra neuronal cells in 6 hydroxydopamine(6 OHDA) induced PD model rats. Results [WT5”,6BZ]Apoptotic cells were observed in the substantia nigra pars compacta(SNpc) in PD rats with TUNEL method and flow cytometry. The apoptotic rate of SNpc was (48 5±5 5)%, (11 0±3 0)% and (6 5±3 0)%, the percentage of sub G1 cells was (57 3±9 7)%, (11 7±3 6)% and (6 5±1 7)% respectively detected by TUNEL method and flow cytometry in 2 weeks,1 and 3 months model rats. The results obtained from the two methods were comparable. The difference of results between 2 weeks and 1 and 3 months model rats was significant( P 0 001). Conclusions The study showed that apoptosis was a main death mode of nigral neuronal cells in 6 OHDA induced PD model rats, which may play a key role in the development of Parkinson's disease.
Publication Year: 2000
Publication Date: 2000-01-01
Language: en
Type: article
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