Title: Oxidative stress of substantia nigral dopaminergic neuron in rat model of parkinson's disease
Abstract: Objective To explore the oxidative stress pathogen esis of Parkinson's disease (PD) rat models in the neurons of substantia nigra.Methods 6-OHDA was stereotacticly injected into the right striatum of the rats at two sites in order to produce P D models.Observed the morphological alterations by striatal and substantia nigr al hematoxylin eosin (HE) stain and compared the activity of superoxide dismutase (SOD),the quantities of malondialdehyde (MDA) and NO x(the metabolism products of nitric oxide) between the injuried tissue and no rmal tissues.Results In the model group of 30 rats,there were 22 successful PD models.Photomicrograph s of HE stain showed that the dopaminergic neurons in the right substantia nigra pars compacta (SNc) were severely lost,and the remaining neurons were atrophic.The level of SOD in the ri ght substantia nigra(SN) of the model group descended significantly,while the quantity of MDA and NO x increased obviously respectively compared with either the sham g roup or the normal control group (P0.05).Conclusion It is rather effective method to make PD rat models using 6-OHDA injected into the striatium of one side at two target sites.In PD rats the acti vitis of SOD are descent,while the quantities of MDA and NO x are increas ed in the dopaminergic neurons of SN.Oxidative stress plays a key role in the pa thogenesis of PD.
Publication Year: 2003
Publication Date: 2003-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot