Title: RP-HPLC analysis of hesperidin,glycyrrhizic acid,honokiol,magnolol and atractylodin in Xiao'er Moji tablets
Abstract: Objective:To develop an HPLC method for determination of hesperidin,glycyrrhizic acid,honokiol,magnolol and atractylodin in Xiao'er Moji tablets.Methods:The analysis was carried out on a DIKMA DIMONSIL C18(4.6 mm×250 mm,5 μm) column;The mobile phases were 80% acetonitrile(A)-aqueous solution containing 0.2% phosphoric acid and 5% acetonitrile(B) with gradient elution(0-10 min,80% B→70% B;10-20 min,70% B→50% B;20-40 min,50% B→5% B;40-50 min,5% B→0% B) and the flow rate was 1.0 mL·min-1;The detection wavelengths were set at 283 nm for hesperidin(0-15 min),250 nm for glycyrrhizic acid(16-30 min),294 nm for honokiol and magnolol(31-40 min),and 335 nm for atractylodin(41-50 min);The column temperature was 40 ℃.Results:The linear ranges of hesperidin,glycyrrhizic acid,honokiol,magnolol and atractylodin were 0.012-3.06,0.009-2.23,0.004-0.992,0.004-0.948,0.0005-0.123 μg(r≥0.9990,respectively);The average recoveries(n=6) were 95.8%(RSD=1.9%),96.4%(RSD=1.2%),98.3%(RSD=1.7%),98.2%(RSD=1.6%),100.4%(RSD=1.3%),respectively.Conclusion:This method is simple and can be used to determine the above 5 components with satisfactory accuracy and repeatability.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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