Abstract: AIM To establish the quality standards for Manyokin Tea(Cassiae Semen,Aurantii Fructus immaturus,Magnoliae officinalis Cortex,Citri Reticulatae Pericarpium,etc.).METHODS Cassiae Semen,Aurantii Fructus immaturus and Magnoliae officinalis Cortex were identified by TLC.A RP-HPLC method was established to determine magnolol and honokiol in Manyokin Tea.The analytical column was DiamonsidTM-C18 column.The mobile phase was methanol-0.3% phosphoric acid(68∶ 32) with the flow rate of 1.0 mL/min.The detection wavelength was set at 294 nm.The column temperature was 30 ℃.RESULTS The TLC spots developed was clear.The linear range of magnolol was 44.4-111.0 μg/mL(r=0.999 3) and that of honokiol was 15.0-45.0 μg/mL(r=0.999 1).The RSDs of the method precision were 1.28% and 1.52%(n=6).The RSDs of stability in 12 h were 0.95% and 1.24%.The average content of magnolol was 0.88 mg/g(RSD was 1.83%),and honokiol was 0.33 mg/g(RSD was 2.30%) in repeatability test.The average recoveries of magnolol and honokiol were 99.2% and 96.2%,respectively.CONCLUSION This method is accurate and reliable,can be used for quality control of this preparation.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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