Title: Simultaneous HPLC determination of five components in Tincture Huoxiang Zhengqi
Abstract: Objective:To develop an HPLC method for the determination of glycyrrhizic acid,imperatorin,isoimper- atorin,magnolol,and honokiol in Tincture Huoxiang Zhengqi.Methods:The method was conducted on an Agilent Zorbax XDB-C_(18) column (250 mm×4.6 mm,5 μm) with 0.05% formic acid (A)-acetonitrile (B) as mobile phase(gradient elution:10 min,40% B;25 min,45% B;30 min,50% B;60 min,55% B),flow rate 1.0 mL· min~(-1),detecting at 254 nm.Results:The linearity ranges of glycyrrhizic acid,imperatorin,isoimperatorin,magnolol and honokiol were 0.094-0.470,0.041-0.205,0.018-0.090,0.235-1.175,and 0.311-1.555 μg,respec- tively;r≥0.9998.The average recoveries (n=6) of them were 98.3 %,99.6%,98.6%,98.0%,and 99.6%,re- spectively.Conclusion:The method is accurate,sensitive and specific for quality control of Tincture Huoxiang Zhengqi.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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