Title: Determination of Scopoletin in Qinghao by HPLC
Abstract:Objective:To develop a method for the determination of scopoletin in Qinghao.Method:Sample was extracted with methanol.Scopoletin in Qinghao was determined by HPLC with ODS column.The mobile phase was...Objective:To develop a method for the determination of scopoletin in Qinghao.Method:Sample was extracted with methanol.Scopoletin in Qinghao was determined by HPLC with ODS column.The mobile phase was methanol-0.08% H_3PO_4(4∶6)solution.The detection wavelength was 340nm.Result:Scopoletin in sample solution was well separated.Linearity of scopoletin was good(r=(0.999 9))in range of 0.021~0.42μg.The average recovery was 99.3%,RSD was 2.0%.Conclusion:The method is simple,accurate,repeatable and suitable for the determination of scopoletin in Qinghao.Read More
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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