Title: Effect and mechanism of artesunate on proliferation and apoptosis in hunman ovarian cancer HO8910 cell line
Abstract: Objective To investigate the effect and mechanism of artesunate(Art) on proliferation and apoptosis of hunman ovarian cancer cell line HO8910 cell.Methods The effect of Art on proliferation of HO8910 cells were observed by MTT asssy.Detection of apoptosis and cell cycle analysis were performed with flow cytometry.The expressions of Bcl- 2and Bax were detected by Western blot.Result The proliferation and apoptosis of HO8910 cells was inhibited by Art in dosage and time dependent manner.The cell cycle was arrested in G_0/G_1 phase in HO8910 cells treated with Art.The expression of Bcl-2 decrease and Bax increased in HO8910 cells treated with Art.Conclusion Art inhibits the proliferation and induces the apoptosis of HO8910 in a dose and time dependent manner.It may be due to down-regulating the expressions of Bcl-2 and up-regulating Bax.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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