Title: Effects of TRAIL on Proliferation,Apoptosis and NF-κB of Primary Human Ovarian Cancer Cell
Abstract: Objective To investigate the regulatory effects of TRAIL on proliferation and apoptosis of primary human ovarian cancer cell in vitro,and the activation and transportation of NF-κB.Methods Primary human ovarian cancer cells were obtained from twelve patients.Each patient's cell was divided to six groups,and treated with TRAIL(5~100μg/L) for 24~96h respectively.The ratio of growth inhibition was measured with MTT in primary human ovarian cancer cells.The ratio of apoptosis and the transportation and activation of NF-κB were measured by immunofluorescence in primary human ovarian cancer cells.Results The ratio of growth inhibition increased in dose-and time-dependent manner when the concentration was 5,10or 20μg/L at 24,48,72h.The ratio of apoptosis increased along with the time lasting.It was(28±1.18)% with concentration of 100μg/L at 24h,and(55± 4.12)%at 96h.The level of NF-κB in nucleus and cytoplasm was highly expressed.Conclusion TRAIL inhibits proliferation and growth of primary human ovarian cancer cell,and induces apoptosis.It enhances conveying the NF-κB from cytolymph to nucleolus.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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