Title: Simultaneous Determination of Three Active Components in Yuanhu Zhitong Tablets by High Performance Liquid Chromatography
Abstract: [Objective] To establish a method for simultaneous determination of protopine, berberine hydrochloride and tetrahydropalmatine in Yuanhuzhitong(Rhizoma corydalis analgesic) tablets. [Methods] The RP-HPLC was used, Kromasil DS-C18(250 mm×4.6 mm, 5 μm) column was adopted at 30℃, taking acetonitrile-acetate buffer solution(pH 6.0) (28∶72) as the mobile phase. The flow rate was 0.8 mL/min and the detection wavelength was 280 nm. [Results] The linear range of protone, berberine hydrochloride and tetrahydropalmatine was at 0.068 48~1.712 μg, 0.010 68~0.356 0 μg, 0.067 92~2.264 μg respectively. The average recovery rate was 99.9%, 99.6%, 100.5% respectively. RSD was 0.82%,1.8%,1.3%(n=6). [Conclusions] The method was convenient and can be used for the quality control of Yuanhuzhitong Tablets.
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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Cited By Count: 2
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