Title: Determination of Tetrahydropalmatine in Complex Yuanhu Zhitong Capsule by HPLC
Abstract: Objective To establish the method for determining the content of tetrahydropalmatine in complex Yuanhu Zhitong capsule by HPLC.MethodsThe separation was performed on Alltima C18 column(5μm,4.6mm×250mm) with methanol-H2O-triethylamine(65∶35∶0.5) as mobile phase.The detection wavelength was at 280 nm,the column temperature was 30 ℃,and the mobile phase speed was 1.0ml per min.ResultsThe calibration curve was linear in the range of 0.1029 to 1.0288 μg for the tetrahydropalmatine content and the linear equation was y=4.840 1x +0.089 5(r=0.999 7).The average recovery rate was 101.17% and the relative standard deviation was 1.02%.ConclusionThe method is simple,rapid,accurate and reproducible.It can be used for the determination of tetrahydropalmatine content complex Yuanhu Zhitong capsule.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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Cited By Count: 2
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