Title: Determination of the Content of Berberine Hydrochloride in Tibetan Medicine Bawei Zhangyacai Pills by HPLC
Abstract: [Objective] To establish a quantitative method to determine the contents of berberine hydrochloride in Tibetan medicine Bawei Zhangyacai Pills.[Method] The quantitative analysis was carried out by HPLC with a C18 column(250 mm × 4.6 mm,5μm).The mobile phase was acetonitrile-0.033 mol/L potassium dihydrogen phosphate(25∶75) with a flow rate at 1.0 ml/min.The detection wavelength was set at 265 nm.[Result] The linear ranges for berberine hydrochloride was 0.165 6-2.318 4μg(r =0.999 5).The average recovery was 97.30%,RSD was 1.57%(n = 6).[Conclusion] This method is simple,rapid and reliable with good reproducibility,and can be used as the determination method for berberine hydrochloride quantity in Bawei Zhangyacai Pills.
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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