Title: Establishment and Evaluation of Mice Model with Kidney Yang Deficiency Infected by Influenza Virus
Abstract:Objective: To establish a mouse model with kidney Yang deficiency infected by influenza virus.Method: Kidney Yang deficiency mice were established by intraperitoneal injection of estradiol benzoate(0....Objective: To establish a mouse model with kidney Yang deficiency infected by influenza virus.Method: Kidney Yang deficiency mice were established by intraperitoneal injection of estradiol benzoate(0.008 mg.g-1) as previously described.The physical signs in mice were observed,and the swimming time of mice,rectal temperature,toe temperature,autonomic mobility were examined.On the basis of above model,Mice were inoculated with mouse lung influenza virus(FM1) by the nasal cavity to establish the mice model with kidney Yang deficiency infected by influenza virus.Then physical signs,mortality,body weight and temperature changes were observed daily.The concentrations of tumor necrosis factor-α(TNF-α) and interferon-γ(IFN-γ) in mouse serum were measured,and the organ indexes of lung,spleen,kidney,adrenal and thymus were detected.Pathological changes of lung were observed.Result: The swimming time,rectal temperature,toe temperature,and autonomic activities of mice model were significantly decreased compared with normal group(P 0.01);Viral pneumonia was detected in lung tissue.Compared with the normal control,the thymus index of model mice was remarkably reduced(P 0.01).The level of IFN-γ was obviously increased(P 0.05) and TNF-α level was decreased.Conclusion: To some extent,the symptoms of kidney-yang deficiency with influenza disease could embodied in this mouse model,which was successfully established by combining estradiol intraperitoneal injection with nasal cavity by influenza virus FM1 and this provided a more ideal experimental model for pathogenesis and drug intervention.Read More
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
Access and Citation
Cited By Count: 3
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot