Title: Protective Effect of Puerarin on Nerve Cells in Substantia Nigra of Parkinson's Disease Rats Induced by 6-hydroxydopamine
Abstract: Objective:To investigate the effect of puerarin on parkinson'as rats model induced by 6-hydroxydopamine(6-OHDA).Method: Parkinson's rats model was established,the rats were randomly divided into 5 groups: model group,L-dopa group(40 mg · kg-1),low-,medium-and high-dosage groups of puerarin(20,40,80 mg · kg-1).The drugs were intragastrically perfused to rats daily for 30 consecutive days.The activity of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px) as well as the contents of MDA in substantia nigra tissue were tested by biochemical method;the pathological change of nerve cells in substantia nigra was observed by HE staining;then the expressions of induced nitric oxide synthase(iNOS),cyclic adenosine monophosphate(cAMP)-response-element-binding-protein(CREB) in substantia nigra were using Western blot analysis.Result: The condition of Parkinson's rats model was alleviated via puerarin treatment.Compared to model control group,puerarin significantly elevated the activities of GSH-Px and SOD in substantia nigra tissue of parkinson'as rats,while the MDA contents was reduced(P0.01);and down-regulated the iNOS protein level in substantia nigra tissue,while the expression of CREB was increased(P0.01).Conclusion: The findings indicate that puerarin has protective effect on the 6-OHDA-induced PD rats the mechanism may be associated with restraining the oxidative stress and regulations of endogenous iNOS,CREB expressions.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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Cited By Count: 4
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