Title: Effect of Puerarin on the Nrf2/ARE pathway in Substantia Nigra Tissue of Parkinson's Rats Induced by 6-hydroxydopamine
Abstract: Objective:To study the effect of puerarin on the Nrf2/ARE pathway in substantia nigra tissue of Parkinson's disease(PD) rats model induced by 6-hydroxydopamine(6-OHDA).Method:SD PD rats model was established,the rats were randomly divided into 5 groups:model group,madopar group(40 mg·kg-1),low-,medium-and high-dosage groups of puerarin(20,40,80 mg·kg-1).The drugs were intragastrically perfused to rats daily for 30 consecutive days.The activities of γ-glutamylcysteine synthetase(γ-GCS),glutathione(GSH)and catalase(CAT)in substantia nigra tissue were tested by ELISA method.The expression of cytochrome c oxidase(COX)mRNA was tested by RT-PCR assay.And the nuclear factor E2-related factor 2(Nrf2),Kelch-like ECH-associated protein 1(Keapl)protein expressions were tested by Western blot analysis.Result:Compared to model group,puerarin effectually elevated the activityof γ-GCS,GSH and CAT in substantia nigra tissue of PD rats(P0.01).And the COX expression at mRNA level in substantia nigra was upregulated(P0.01).The expression of Nrf2 and Keapl proteins was markedly increased.Conclusion:The results reveal that puerarin effectively fight against the oxidative stress damage in nerve cells in substantia nigra of PD rats induced by 6-OHDA,and its probable mechanism may be involved in regulation of Nrf2/ARE pathway.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot