Title: Construction of Eukaryotic Expression Vector for Antisense Vascular Endothelial Growth Factor_(165) cDNA and Its Inhibitory Effect on Growth of Breast Cancer Cells
Abstract: Objective To construct the eukaryotic expression vector for antisense vascular endothelial growth factor 165 (VEGF165) cDNA and study the inhibitory effect of antisense VEGF165 cDNA on the growth of breast cancer cells. Methods Amplify human VEGF165 cDNA from breast cancer MCF-7 cells by RT-PCR and inversely insert into eukaryotic expression vector pcDNA3. Transfect MCF-7 cells with the constructed recombinant plasmid pcDNA3 / anti-VEGF165 and observe the expression of VEGF165 and cell cycle before and after transfection. Results Sequencing result proved that recombinant plasmid pcDNA3 / anti-VEGF165 was constructed correctly. After transfection with the recombinant recombinant plasmid, both the expression level of VEGF165 as well as survival rate and proliferation ability of MCF-7 cells decreased, and the percentage of MCF-7 cells at G1 phase increased while that at S phase decreased. Conclusion The eukaryotic expression vector for antisense VEGF165 cDNA was successfully constructed, which inhibited the growth of breast cancer MCF-7 cells significantly.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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