Title: Effect of P1N1 antisense nucleotide on proliferation of human breast cancer cell line MCF-7
Abstract: Objective To observe the effect of Pin1 antisense nucleotide on cell proliferation of human breast cancer cell line MCF-7.Methods The eukaryotic vector named pPINas,expressing Pin1 antisense nucleotide,was constructed.MCF-7 was transfected with pPINas and selected in the culture with G418.The selected clone MCF-7PINAS was checked for expression of Pin1 by RT-PCR and Western blot.The proliferation of cells in clone MCF-7PINAS were investigated by MTT.Results The expression of Pin1 at mRNA and protein level in MCF-7PINAS clone was down-regulated remarkably.MTT showed the proliferation of MCF-7PINAS was significantly retarded contrasting to MCF-7(P0.05).Conclusion Blocking the expression of Pin1 with antisense nucleotide can signficntly depress the proliferation activity of MCF-7 to have offered new insight in gene research and treatment of human breast cancer.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot