Title: The effect of PRLR antisense RNA on proliferation of human breast cancer cell MCF-7
Abstract: Objective:To construct eukaryotic expression vector carrying anti-sense PRLR gene and investigate whether the reco-mbined plasmids could regulate the expression of PRLR mRNA and affect the growth of human breast cancer cell line MCF-7.Methods:PRLR cDNA was inserted into the expression plasmid pcDNA3.1(-)to construct the recombined plasmids p-anti-PRLR that encoding PRLR cDNA in an anti-sense orientation,then the recombined plasmids transfected MCF-7 cells by lipofectin.The stable cell lines were obtained after G418 selection.PRLR mRNA expression was detected by real-time PCR.MTT assay,clone formation test,and the flow cytometry(FCM)were used to evaluate cell proliferation.Results:(1)The eukaryotic expression vector p-anti-PRLR was constructed successfully.(2)Antisense PRLR gene decreased the level of PRLR mRNA in MCF-7 cells.(3)After antisense gene transfection,the proliferation ability of MCF-7 cell was decreased,and the G1 phase cells were increased,but the S phase cells were decreased in cell cycle.Conclusion:The results suggested that antisense PRLR could inhibit MCF-7 cell proliferation,and it might be significant for the breast cancer gene therapy.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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