Title: Transfection of Antisense Human Transforming Growth Factor-α (TGF-α) Complementary DNA Reduces Growth Rate of a Human Non-Small Cell Lung Cancer Cell Line
Abstract: The proliferation of human non-small cell lung cancer (NSCLC) cells was demonstrated of being regulated by TG F-α-stimulated autocrine loop. The present study was designed by transfecting NSCLC cell line, NCI-H460, with construct of human coding fragment TGF-α cDNA in the antisense orientation that is the transcriptionally controlled by a strong eukaryotic promoter. The expressed antisense TGF-α transcript is to neutralize endogenous TGF-α expression. The growth rate of the cloned cells H460 with antisense construct selected for G418 resistance were shown reduced compared with those that were transfected with vector alone and the parental cells. In addition, TGF-α transcript level was observed reduced in the cells with antisense TGF-α construct. The result of this work provides a good model for studying the effects of reduction in endogenous TGF-ex expression by transfecting antisense construct on anchorage-dependent cell growth in vitro and on tumorigenesis in vivo.
Publication Year: 1997
Publication Date: 1997-06-01
Language: en
Type: article
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