Title: Expression of tumor necrosis factor-α and nuclear factor-κB and their correlationin hippocampus of rats with status epilepticus
Abstract:Objective To investigate the expression of tumor necrosis factor-α(TNF-α) and nuclear factor(NF-κB) in hippocampus of rats with status epilepticus(SE). MethodsTwenty Wistar rats were intraperitoneally...Objective To investigate the expression of tumor necrosis factor-α(TNF-α) and nuclear factor(NF-κB) in hippocampus of rats with status epilepticus(SE). MethodsTwenty Wistar rats were intraperitoneally injected with 10% pilocarpine(300 mg/kg)(SE group),and injection was ceased 60 min after SE onset.Besides,six rats treated with same amount of normal saline were served as control group.Hippocampus tissues of rats were obtained 24 h after treatment,the number of neurons in CA3 area of hippocampus was observed with HE staining,the expression of NF-κB and TNF-α in hippocampus tissues was determined by Western blotting and ELISA respectively.Pearson correlation analysis was adopted to explore the correlation between the expression of TNF-α and that of NF-κB. ResultsThe number of neurons in CA3 area of hippocampus in SE group was much smaller than that in control group(53.26±2.67 vs 103.22±0.83,P0.001).The expression of TNF-α and NF-κB in hippocampus tissues in SE group was significantly higher than that in control group(P0.001,P0.05),and there was a positive correlation between the expression of TNF-α and that of NF-κB in SE group(r=0.457,P=0.02). ConclusionThe expression of TNF-α and NF-κB in hippocampus tissues of rats increases 24 h after SE,and there is a positive correlation between the expression of TNF-α and that of NF-κB.Read More
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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