Title: [Effect of cadmium choride on oxidative damage and expression of NF-κB in brain tissue of mice].
Abstract: To investigate the oxidative damage induced by cadmium choride and the expression of nuclear factor κB( NF-κB), tumor necrosis factor-α( TNF-α) and intercellular cell adhesion molecule-1( ICAM-1) in brain tissue of mice.A total of 32 adult ICR mice were divided into 4 groups randomly including CdCl_2 2. 0, 1. 0 and 0. 5 mg/kg and NS control group, all mice were through intraperitoneally injected administration for 14 days, after the last exposure, open-field test were used and all mice were sacrificed by cervical dislocation, the expression of reactive oxygen species( ROS)was measured and the expression of TNF-α, ICAM-1 were observed by ELISA, the level of NF-κB P65 in the brain were detected by immunohistochemistry and western blot, the slices of the brain were stained with HE for histopathological examination.Thebody weight gain in CdCl_22. 0 mg/kg was significant lower than others( P < 0. 05), the number of crossing lattice in CdCl_2 2. 0 mg/kg was higher than the NS control group in the open field test. The expression of ROS increased in CdCl_2 2. 0 and 1. 0 mg/kg groups( P < 0. 05), the expression of TNF-α increased in CdCl_2 2. 0 mg/kg group( P < 0. 05), no obvious change was found among every group of ICAM-1, the expression of NF-κB P65 in the brain increased in cadmium 2. 0 mg/kg group. The neuropathological test showed that the cerebral cortex and hippocampal cells were more loose in 2. 0 mg/kg group than the control.The level of ROS in mouse brain tissue increased, NF-κB pathway was activated by ROS and the expression of inflammatory cytokine TNF-α was increased.
Publication Year: 2017
Publication Date: 2017-09-01
Language: en
Type: article
Indexed In: ['pubmed']
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