Title: Establishment of adventitious bud regenerating system from in vitro Euonymus fortunei
Abstract: An efficient adventitious bud regeneration system of E.fortuneiwas established when inter-node and hypocotyls segments were used as explants.The optimum medium for induction of adventitious buds from inter-node of E.fortunei was MS+6-BA 1.5 mg/L +IBA 0.1 mg/L,and the regeneration rate of adventitious buds reached 82.6%.For hypocotyls explants,50 d pre-culture and 6-BA at 1.3 mg/L to 1.5 mg/L were efficient to adventitious buds induction.The perfect medium for buds regeneration from hypocotyls segments was MS+6-BA 1.5 mg/L+ NAA 0.1 mg/L,and the regeneration rate was 95.7%.Perfect results were also achieved when sugar or sucrose was used as carbon source.The regeneration rate reached 100% when glucose was used as carbon source,but all of the regenerated buds were hyperhydration.Adventitious roots were induced successfully when the shoots were cultured on half-strength MS media supplemented with 0.7 mg/L IAA(92.9%).More than 90% of regenerated plantlets were successfully transplanted after acclimation.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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