Abstract: The mature embryo of Pinus bangeana was used as explants to induce adventitious buds and form plantlets.The results showed that the best medium for adventitious bud inducement is MS adding with(3.56) mg/L 6-BA and 0.294 mg/L NAA for a nearly 100% induction rate.The mean proliferation rate of adventitious bud reaches(6.3) in MS adding with 0.05 mg/L NAA.The highest proliferation rate(10) of adventitious bud appeared on SH adding with 0.05 mg/L NAA and 4 mg/L 6-BA.The additional activated charcoal and GA_3 were capable of enhancing the growth of adventitious buds;The ratio of effective shoot(≥2 cm and ≥4 cm) increased as the concentration of activated charcoal and GA_3 increasing,the growing and elongation of adventitious buds were restrained when the concentrations of activated charcoal and GA_3 exceeded 2.75 g/L and 4.1 mg/L respectively.The rootless plantlets were successfully regenerated from adventitious buds.
Publication Year: 2006
Publication Date: 2006-01-01
Language: en
Type: article
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