Title: Influence of soluble receptor for advanced glycation end products on hypoxia/reoxygenation myocardial mitochondrial membrane potential
Abstract: Objective To observe the influence of soluble receptor of advanced glycation end products(sRAGE) on hypoxia/reoxygenation(H/R) myocardial pathway of mitochondria-apoptosis.Methods Neonatal rats cardiomyocytes were cultured for 72 h.The H/R injury model was replicated by cardiomyocytes hypoxia 3 h/ reoxygenation 2 h.The cells were randomly divided into four groups: control group,control+sRAGE group,H/R group,and H/R+sRAGE group.Changes of mitochondrial membrane potential were detected by fluorescent probe called JC-1,esterification calcein and cobalt chloride were incubated to detect the opening of the mitochondrial permeability transition pore(mPTP),the myocardial apoptosis was detected with Westernblot.Results Compared with control group,H/R group could increase the opening of mPTP,the degree of depolarization of mitochondrial membrane potential and the rate of apoptosis(all P0.05).Compared with H/R group,H/R-sRAGE group could reduce the opening of mPTP,lower the degree of depolarization of mitochondrial membrane potential and decrease the rate of apoptosis(all P0.05).Conclusions sRAGE may inhibit the pathway of mitochondria-apoptosis to antagonize myocardial H/R injury.
Publication Year: 2013
Publication Date: 2013-01-01
Language: en
Type: article
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