Title: Effect of glycine liposomes on mitochondrial membrane potential and apoptosis in cultured cardiomyocytes
Abstract: AIM:To observe the effect of glycine liposomes on the mitochondrial membrane potential and the apoptosis rate in cardiomyocytes induced by hypoxia/reoxygenation injury.METHODS:A cardiomyocyte injury model was established by using hypoxia/reoxygenation.DiOC6(3)as fluorescence molecular probe was used to detect the mitochondrial membrane potential in each group.The method of Annexin V associated with PI was used to detect the apoptosis ratio in each group.RESULTS:(1)The result of flow cytometry showed that the mitochondrial membrane potential of cardiomyocytes in H/R group was obviously lower than that in control group(P0.01).The decrease in mitochondrial membrane potential in Gly-liposome group was the lowest,the percentage of cells about the part of hypofluorescence was(9.61±0.76)%,which was lower than that in glycine group(P0.01).(2)The apoptosis rate of cardiomyocytes in H/R group was higher than that in control group(20.78±1.58)%,P0.01.After the treatment of Gly-liposome,the apoptosis rate of cardiomyocytes was lower than that in glycine group(P0.01).No difference in the apoptosis ratios between blank-liposome group and H/R group was observed(P0.05).CONCLUSION:Glycine liposomes protect cultured cardiomyocytes against hypoxia/reoxygenation injury.Glycine liposomes produce the better protective effects than glycine.
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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