Title: Antiapoptotic effects and mechanism of pinacidil,metoprolol, glutamine,insulin single and combination on H9c2 cardiac myocytes exposed to simulated hypoxia/reoxygenation
Abstract: Aim To investigate protective effects and mechanism of pinacidil,metoprolol,glutamine,insulin on H9c2 cardiac myocytes exposed to simulated hypoxia/reoxygenation.Methods H9c2 cardiac myocytes were randomly divided into seven groups:① Control group;② Hypoxia/reoxygenation(H/R) group;③ Pinacidil(Pin) group;④ Metoprolol(Met) group;⑤ Glutamine(Glu) group;⑥ Insulin(Ins) group;⑦ Pinacidil+Metoprolol+Glutamine+Insulin(PMGI) group.The survival rates of cardiomyocytes were detected.The activity of lactate dehydrogenase(LDH) in the culture medium was measured.The mitochondria were labeled by Rhodamine123 fluorescence probe and the fluorescent intensity produced by Rh123 was measured,which reflected changes of mitochondrial membrane potential(MMP,△Ψm).The apoptotic percentage was measured with flow cytometry.Results The combined use of pinacidil,metoprolol,glutamine,and insulin could protect the H9c2 cardiac cell membrane caused by hypoxia/reoxygenation injury,increase cell survival rate,decrease LDH leakage,decrease the apoptotic rate and increase the mitochondrial membrane potential.Conclusions Compared to the individual drug,the combination of the different mechanisms protects the mitochondria of cardiac myocyte,decreases the injury and enhances antiapoptotic effection of myocardial cells.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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