Title: Determination of Ursolic Acid Content in Photinia serrμlate Grown in Different Regions by HPLC
Abstract: Objective To establish an HPLC method for determination of ursolic acid content in Photinia serrμlate Lindl.Methods Kromasil Lnertex C18(5 μm,250 mm×4.6 mm) column was used with a mobile phase of methanol-water-glacial acetic acid-triethanolamine(90∶ 10∶ 0.04∶ 0.02).The column temperature was 16 ℃.Results The content was detected at a wavelength of 210 nm.The flow rate was 1.0 ml · min-1.The calibration curve was linear within the range of 0.41-3.28 μg(r=0.9999).The average recovery was 100.0% and RSD=1.3%.Conclusion This method is highly reproducible and accurate.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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