Title: HPLC determination of rupestric acid in Artemisia rupestris L.
Abstract: Objective:To develop an HPLC method for determination of rupestric acid in Artemisia rupestris.Methods:Rupestric acid was separated by DiamonsilTM C18(4.6 mm×250 mm,5 μm)column with methanol-water(2.5% acetic acid)(1∶1) as mobile phases at a flow rate of 1 mL·min-1;The detection wavelength was 244 nm,and the column temperature was 25 ℃.Results:The linear range of rupestric acid was in 0.158-0.948 μg;The average recovery(n=5) was 97.36%,RSD=1.5%.Conclusion:The method is simple,accurate and reliable.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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