Title: Simultaneously Determination of Oleanolic Acid and Ursolic Acid in Medofenoxate Fruits by HPLC
Abstract: Objective To establish a HPLC method for the simultaneous determination of Oleanolic Acid and Ursolic Acid in Medofenoxate fruits.Methods Agilent XDB C18(4.6 mm×250 mm,5μm) column was applied,using methanol-acetonitrile-0.3% phosphoric acid(30 60 10) as mobile phase with the rate of 0.8mL·min-1.The column temperature was room temperature and the detective wavelength was 210nm.Results A good linearity was obtained over the range of 0.2052~3.2832μg for Oleanolic Acid(r=0.9995).The average recovery(n=6) was 101.11%(RSD=1.69%);A good linearity was obtained over the range of 0.2072~3.3152μg for Ursolic Acid(r=0.9993).The average recovery(n=6) was 98.51%(RSD=2.67%).Conclusion The method is practical,convenient,accurate,and can be used as the simulta-neous determination of Oleanolic Acid and Ursolic Acid in Medofenoxate fruits.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot