Title: Quality Control Standard for Xuelianhuangtong Capsules Against High-altitude Hypoxia
Abstract: Objective To establish the quality control standard for Xuelianhuangtong capsules against high-altitude hypoxia. Methods The Rutin and Chlorogenic Acid were identified by thin layer chromatography(TLC); total flavonoids content were determined by ultraviolet spectrophotometry(UV),and Rutin content was determined by high performance liquid chromatography(HPLC). The Hypersil ODS2 C18(150 mm ×4. 6 mm,5 μm) column was used,and the mobile phase consisted of methanol-water(40∶ 60),and the flow rate was 1 ml /min; the column temperature was room temperature,and the detection wavelength was at 340 nm. Results The spots in the TLC were clear with good disassociation effect; the good linear relationship was achieved within the range of total flavonoids content of 9. 84μg /ml-59.04μg / ml(r = 0. 9997),and its average recovery ratio was 99. 32% with 1. 47% of RSD(n = 6); the good linear relationship was achieved within the range of Rutin content of 4-120 μg /ml(r = 0. 9999),and its average recovery of application of sample was 97. 98% with 1. 35% of RSD(n = 6). Conclusion The method is simple,practical and accurate with good repeatability,so it can be used for the quality control of Xuelianhuangtong capsules.
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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