Title: Determination of the Daidzein Capsules by HPLC
Abstract:A HPLC method was established to determine the content of daidzein capsules.METHODS: Chromatographic condition included the Hypersil ODS column(4.6 mm ×150 mm,5 μm) and mobile phase consisting of wate...A HPLC method was established to determine the content of daidzein capsules.METHODS: Chromatographic condition included the Hypersil ODS column(4.6 mm ×150 mm,5 μm) and mobile phase consisting of water-acetonitrile(70∶30),The flow rate was 1.0 mL·min-1,the detection wavelength was set at 249 nm,the column temperature was 30 ℃.RESULTS: The linear calibration curves were obtained in the concentration range of 1.0~20.0 μg·mL-1(r = 0.9995).The mean recovery was 101.98%,RSD=1.45%(n=9),and good precision.CONCLUSION: This method is sensitive,accurate and high selectivity.The method has been used to determine the dissolution of this dosage form with satisfactory results.Read More
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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