Title: Apoptosis of Human Umbilical Vein Endothelial Cells Induced by Trans C18∶1
Abstract:The target of this study is to exoplore the apoptotic mechanism of endothelial cells through detecting relevanting apoptosis index.For this,EC were cultured with trans C18∶1 at different concentration...The target of this study is to exoplore the apoptotic mechanism of endothelial cells through detecting relevanting apoptosis index.For this,EC were cultured with trans C18∶1 at different concentrations,then the morphological changes of endothelial cells were observed through giemsa staining.The apoptotic cells were detected by flow cytometry.The activity of caspase-3 was detected by spectrophotography after endothelial cells treated with trans C18∶1.The cell viability was measured by MTT after endothelial cells cultured with trans C18∶1 and caspase inhibitor for 24~48 h.Results: The number of apoptotic cells raised and endothelial cells displayed classical apoptotic morphology after endothelial cells cultured with trans C18∶1.The activity of caspase-3 ascended and the caspase inhibitor could protect endothelial cells from trans C18∶1.Read More
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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