Title: Application of phage-lysis method in clinical detection of drug resistance of Mycobacterium tuberculosis
Abstract:Objective To assess the value of phage-lysis method in clinical detection of drug resistance of Mycobacterium tuberculosis.Methods The resistance of Mycobacterium tubercuolosis from 108 clinical isola...Objective To assess the value of phage-lysis method in clinical detection of drug resistance of Mycobacterium tuberculosis.Methods The resistance of Mycobacterium tubercuolosis from 108 clinical isolated samples to rifampin,isoniazid,streptomycin,and ethambutol were detected with phage-lysis method and comparing with the results of concentration ratio(CR)method's.Results The susceptibility,specificity and accuracy of PL method in detection of resisthnace of Mycobacterium tuberculosis to rifampin were 100%,96.0%,and 96.3%;to isoniazid were 87.5%,95.7%,94.4%;to streptomycin 76.2%,100%,95.4% and to ethambutol were 75.0%,96.0%,94.4%.Coincidence of the results detected by CR and PL methods were 96.3%(rifampin),94.4%(isoniazid),95.4%(streptomycin),94.4%(ethambutol).Conclusion Comparing with CR methods,PL method should be as the better choice for detecting drug resistance of Mycobacterium tuberculosis as it is simple fast and without requiring sophisticated facilities and allowed application in general laboratories or those at grassrots levels.Read More
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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