Title: Resistant Analysis and Countermeasures in Clinical tuberculosis treatment
Abstract: Objective To make a reasonable choice in understanding the gene mutation rate and resistance to anti-tuberculosis drugs from isolation of patients with pulmonary resistant Mycobacterium tuberculosis.Methods Sputum smear positive governance of new tuberculosis patients and re-treated ones were conducted sputum mycobacterium tuberculosis culture;positive mycobacterium using high and low concentration and testing four anti-tuberculosis drugs resistance,to detect the resistant gene and the mutation rate of mycobacterium tuberculosis using real-time PCR.Results Resistant rate of 127 cases sputum culture positive was 14.2%,resistant rate of initial treatment was 8.1%,that was 35.7% for retreated;Drug resistance rates of four anti-tuberculosis were 8.7% of isoniazid,rifampicin 2.4%,2.4% streptomycin,ethambutol 0.8%;The mutations rates of katG mutations in rpoB rate in resistance gene test in the untreated group was 12.1%(12/99) and 10.1%(10/99),The mutations rates of katG mutations in rpoB rate in retreated group was 32.1%(9 / 28) and 21.4%(6 / 28).Conclusion Mycobacterium isolated from patients with pulmonary tuberculosis in the initial treatment has deveioped resistance,and drug therapy may increase their resistance.The results show that analysis of mycobacterium tuberculosis of drug resistance and resistance to genetic testing can play practical significance in anti-tuberculosis treatment before and during the treatment.
Publication Year: 2007
Publication Date: 2007-01-01
Language: en
Type: article
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Cited By Count: 1
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