Title: Rapid Detection of katG Gene of Resistant Mycobacterium Tuberculosis in Sputum and Isoniazid-resistant Mechanism
Abstract:Objective To rapidly detect katG gene of Mycobacterium tuberculosis in sputum and to explore isoniazid(INH)-resistant mechanism.Methods katG fragment was amplified from M.tuberculosisstrains from sput...Objective To rapidly detect katG gene of Mycobacterium tuberculosis in sputum and to explore isoniazid(INH)-resistant mechanism.Methods katG fragment was amplified from M.tuberculosisstrains from sputum specimens of drug-resistant inpatients or clinic isolates by PCR.The fragments of katG were sequenced.The difference of katG gene fragments sequences of clinical isolates among multi drug-resistant,all drugs-resistant,multiple drug-resistant but INH-susceptible,all drugs-susceptible isolates of M.tuberculosis,or standard strain were compared.Results Within six hours,katG gene of M.tuberculosisstrains from sputum specimen of drug-resistant pulmonary tuberculosis was detected.A 273bp fragment of katG was amplified from three multidrug-resistant strains,two all drugs-resistant strains,one multiple drug-resistant but INH-susceptible isolate,or all drugs-susceptible clinical isolates and H37Rv standard strain of M.tuberculosisstrains.By comparative analysis of gene sequence,spot mutation was found from two multidrug-resistant,two all drugs-resistant strains in M.tuberculosisstrains.Nucleotide C at 2066 was mutated to G.No mutation in H37Rv standard strain,one multidrug-resistant strain,one multiple drug-resistant but INH-susceptible isola or all drugs-susceptible isolate was detected.Conclusion katG gene and its mutation in M.tuberculosis strains could be rapidly and accurately identified by polymerase chain reaction and sequence analysis.INH-resistance was related with spot mutation of katG gene in M.tuberculosisstrains,but other mechanisms may need explored.Read More
Publication Year: 2008
Publication Date: 2008-01-01
Language: en
Type: article
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