Title: Analysis of katG gene mutations in M tuberculosis isolates by direct sequencing
Abstract:To understand the mutations of katG gene in M.tuberculosis isolates,and to evaluate their clinical value.Analyzing the katG genes in 92 M.tuberculosis clinical isolates with PCR,PCR SSCP and PCR D...To understand the mutations of katG gene in M.tuberculosis isolates,and to evaluate their clinical value.Analyzing the katG genes in 92 M.tuberculosis clinical isolates with PCR,PCR SSCP and PCR DS.Mycobacterium tuberculosis strain H37Rv was used as a control. Of 23 drug sensitive isolates,2 displayed abnormal katG SSCP profile.Of 36 non isoniazid resistant isolates,16 had abnormal katG SSCP profile.Of 33 isoniazid resistant isolates,17 were resistant highly to isoniazid,in which 4 katG genes were not amplified and 5 displayed abnormal katG SSCP profile;16 were resistant lowly to isoniazid,in which 13 exhibited abnormal katG SSCP profile.All isolates with abnormal katG SSCP profile had point mutations at codon 315,in which one was AGC→ACC mutation,the others were AGC→AAC mutations .The mutations of katG gene were the most important isoniazid resistant molecular mechanisms in M.tuberculosis.The most common mutations at codon 315 induced the lower MIC for isoniazid.Read More
Publication Year: 2000
Publication Date: 2000-01-01
Language: en
Type: article
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Cited By Count: 1
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