Title: Construction of DNA Fingerprint Database Based on SSR Marker for Varieties(Lines) of Cucumis melo L.
Abstract: Objective) DNA fingerprinting database of varieties of melon (Cucumis melo L.) was constructed and the identification method was obtained. (Method)The simple sequence repeat (SSR) primers were selected using 20 representative melon varieties (lines) for establishing fingerprints of 105 different varieties (lines). (Result)Out of the 1 219 pairs of SSR primers, 18 pairs were screened to generate polymorphic fingerprints among 105 varieties or inbred lines. The results showed that each pair of the primers could detect 4-14 polymorphic bands with an average of 9. The average polymorphism information content (PIC) was 0.68 with a range of 0.55-0.82. The genetic similarity coefficient of the 105 accessions ranged from 0.70 to 0.99. The fingerprint data of all the 18 pairs of core SSR primers were employed to establish the fingerprinting database to distinguish the 105 samples and every accession had its unique fingerprinting. (Conclusion) The results indicated that SSR marker is suitable to construction of DNA fingerprinting database of melon varieties and the database could provide reference for melon identification.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
Access and Citation
Cited By Count: 5
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