Title: Genetic Background Analysis of Several Guizhou Local Citrus Varieties Based on SSR Markers
Abstract: The genetic background of 5local citrus varieties originating from Guizhou Province was analyzed by SSR marker technology.A total of 92 alleles amplified from 24 SSR primer pairs were detected in the 5local varieties and other 14 citrus cultivars used for comparison of DNA fingerprinting.The number of alleles per SSR marker ranged from 2to 7with an average of 3.8,and the allelic polymorphism information content(PIC)values varied from 0.36 to 0.78 with an average of 0.49.DNA fingerprinting showed that 8characteristic bands were amplified using 5of those SSR primer pairs,and they could be used as reference markers for the identification of those local varieties.The similarity coefficient was calculated using the NTSYS software,and UPGMA cluster analysis showed that the 5local varieties could be divided into5 different subgroups at the genetic similarity coefficient of 0.80.Of the five local varieties,Migan and Niurouhongjinju were grouped into different subclusters and each of them demonstrated a unique genotype.Tugan was closely related to Zoupigan.Large-type Bingtang orange and Bingtang orange had the same genetic background.The genetic background of Hongxiang pummelo and Chandler were almost exactly the same.The available data herein provided a reference to the conservation and utilization of those accessions.
Publication Year: 2015
Publication Date: 2015-01-01
Language: en
Type: article
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