Title: Key factors influencing rooting of stem cuttings and dynamics of rooting related oxidases of Camellia oleifera and C.meiocarpa
Abstract: Effects of growing media,cutting types and exogenous auxins on rooting ability of stem cuttings of Camellia meiocarpa and C.oleifera were studied.The dynamic changes of activities of peroxidase(POD),polyphenol oxidase(PPO) and indoleacetic acid oxidase(IAAO) during rooting process were also investigated.The results showed that growing media,cutting types and exogenous auxins had obvious effects on rooting ability and root development of C.meiocarpa and C.oleifera cuttings.Different rooting rates and root development were caused by different growing media,and the mixed media of 75% red soil+25% river sand led to the highest rooting rate(92.6%-93.7%),but the roots were underdeveloped.While the 100% perlite medium had the best rooting development and lower rooting rate(64.2%-66.8%).The cuttings without leaves didn′t root at all,the cutting with half leaves rooted in higher rate than that with complete leaves rooted.Concerning the rate to rooting,top cutting and soft-branch were better than non-top and hard-branch.Compared to No.6 ABT,IBA and NAA,No.3 ABT was the best exogenous auxin,with appropriate concentration of 100 mg · L-1.After cutting,the POD,PPO and IAAO activities changed in the cuttings of C.meiocarpa during rooting process regularly.POD activities showed double peak curve of rise,fall,rise sharply and down,while PPO and IAAO activities showed single-peak curve of rise-down.Compared to the control,the peak of curve of the 3 oxidases obviously increased and appeared ahead.It was concluded that No.3 ABT directly affected the rooting ability of C.meiocarpa.
Publication Year: 2012
Publication Date: 2012-01-01
Language: en
Type: article
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