Title: Comparative Study of 4 Active Constituents in Huanglian Jiedu Decoction before and after Mixing
Abstract: OBJECTIVE:To establish a method for simultaneous determination of jasminoidin,berberine hydrochloride,palmatine hydrochloride and baicalin in Huanglian jiedu decoction(HLJDD),and to explore the influence of compatibility on their contents.METHODS:RP-HPLC method was adopted.The samples were separated on a Diamonsil-C18(250 mm×4.6 mm,5 μm) column with acetonitrile-0.1% glacial acetic acid as mobile phases(gradient elution) at flow rate of 1.0 mL·min-1.The column temperature was at 40 ℃ and the detection wavelength was 260 nm.RESULTS:The linear ranges of jasminoidin,berberine hydrochloride,palmatine hydrochloride and baicalin were 0.125 0~0.625 0 μg,0.050 0~0.250 0 μg,0.037 5~0.187 5 μg,0.045 0~0.225 0 μg,respectively(r=0.999 3,0.999 2,0.999 4 or 0.999 4).Average recoveries were 97.54%,96.07%,104.00% and 96.79%.RSDs were 0.78%,0.78%,0.74%,0.83%(n=6).The contents of 4 active components in HLJDD were all lower than those of same formula dose of single component decoction.CONCLUSION:The contents of chemical compositions in HLJDD are not a simple addition of their contents in single medicine,but have their own internal compatibility law.This article provides a reference for the study of compatibility regularity of HLJDD.
Publication Year: 2011
Publication Date: 2011-01-01
Language: en
Type: article
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