Title: [Determination of 3 kinds of components in huanglian jiedu decoction by HPLC under different UV wavelengths].
Abstract: To determinate 3 kinds of effective components (5 compounds) in Huanglian Jiedu decoction at the same time, namely berberine, palmtine, jatrorrhizine, baicalin and geniposide.A HPLC method detected by 3 different UV wavelengths 345 nm for berberine, palmtine and jatrorrhizine, 280 nm for baicalin, 238 nm for geniposide. Diamonsil C18 (4.6 mm x 250 mm, 5 microm) column was used, the mixture of H2O (A) , CH3OH (B) and 0.05% H3PO4 solution (C) as mobile phase in gradient mode. The column temperature was 35 degrees C and the flow rate was 1 mL x min(-1).This method was applied to determined 5 compounds in Huanglian Jiedu decoction rapidly and accurately.The quality control method is convenient, reasonable and credible for Huanglian Jiedu decoction.
Publication Year: 2006
Publication Date: 2006-10-01
Language: en
Type: article
Indexed In: ['pubmed']
Access and Citation
Cited By Count: 2
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot