Title: Protective Effects of Adenovirus-mediated Heat Shock Protein 70 on Neurons and Glial Cell Injury Induced by Oxidative Stress
Abstract: Objective To investigate the protective effects of recombinant adenovirus-mediated heat shock protein 70(HSP70) on neurons and glial cell injury induced by oxidative stress.Methods Cultured neurons and glial cells were divided into groups A(vAd-HSP70 infected,carrying whole long human HSP70 gene),B(vAd-GFP infected) and C(non-infected).After cells were treated by 0.5 mmol / L hydrogen peroxide(H_2O_2),cell viability was detected by MTT,lactate dehydrogenase(LDH) activity in cell culture supernatant by LDH detection kit,the changes of cell ultrastructure observed with transmission electron microscopy.Results The expression of ectogenous HSP 70 gene was detected by RT-PCR and Western blotting in group A.Cell viability of group A was significantly higher than that of groups B,C after H_2O_2 treatment(P0.01).LDH activities in cell culture supernatant of groups A,B,C were 976±106,1 332±197,1 380±121,respectively,the difference was significant(P0.01).The results of transmission electron microscopy showed that the cytomembrane of group A was more complete and clearer than the other 2 groups,without any irreversible injuries such as obvious mitochondrial swelling or nuclear dissolution.Conclusion The expression of adenovirus-induced exogenous HSP 70 gene can prevent neurons and glial cells from H_2O_2 injuries,with obvious cell protective effects.
Publication Year: 2010
Publication Date: 2010-01-01
Language: en
Type: article
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