Title: Overexpression of heat-shock protein 20 in rat heart myogenic cells confers protection against simulated ischemia/reperfusion injury
Abstract: Aim:To explore whether overexpression of the small heat shock protein HSP20 in rat cardiomyocytes protects against simulated ischemia/reperfusion(SI/R)injury. Methods:Recombinant adenovirus expressing HSP20 was used to infect rat H9c2 cardiomyocytes at high efficiency,as assessed by green fluorescent protein.H9c2 cells were subjected to SI/R stress;survival was estimated through assessment of lactate dehydrogenase and cell apoptosis through caspase-3 activity.Results: Overexpression of HSP20 decreased lactate dehydrogenase release by 21.5% and caspase-3 activity by 58.8%.Pretreatment with the protein kinase C inhibitor Ro- 31-8220(0.1 μmol/L)for 30 min before SI/R canceled the protective effect of HSP20. The selective mitochondrial K+awP channel inhibitor 5-hydroxydecanoate(100 μmol/L) had a similar effect.However,the non-selective K~+_(ATP)channel inhibitor glibenclamide(100 μmol/L)had no significant effect.Conclusion:These data indicate that the protective effect of HSP20 in vitro is primarily due to reduced necrotic and apoptotic death of cardiomyocytes,possibly via the protein kinase C/mitochondrial K~+ATP pathway.
Publication Year: 2005
Publication Date: 2005-01-01
Language: en
Type: article
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