Title: Effect of minocycline on the expression of glial cell line-derived neurotrophic factor α1 in rats with Parkinson's disease
Abstract: Objective To explore the expression of glial cell line-derived neurotrophic factor α1( GFRα1) in substantia nigra and striatum of rats with Parkinson's disease( PD) at different time points and the protective effects of minocycline on PD. Methods One hundred and forty-four health female clearing Sprague-Dawley rats were randomly divided into normal group,sham operation group,model group and minocycline group,with 36 rats in each group. The PD model was estabilished by injecting 6-hydroxydopamine into the substantia nigra and striatum of rats in model group and minocycline group. The minocycline group was given minocycline 45 mg·kg- 1by intragastric administration at 14 d after modeling. The ethology change was evaluated by modified neurological severity score( mNSS). The immunohistochemical method was used to detect the relative expression of GFRα1 in substantia nigra and striatum of rats in each group. Results The mNSS score of rats in normal group and sham operation group was 0 points; there were 33 rats with severe damage and three rods with noderate damage in model group;while there were 31 rats with moderate damage,five rats with mild damage in minocycline group. There was no statistic difference of GFRα1 expression in substantia nigra and striatum of rats between the normal group and sham operation group( P 0. 05); the expression of GFRα1 in substantia nigra and striatum of rats at each time point in model group and minocycline group was significantly lower than that in normal group and sham group( P 0. 05); compared with model group,the expression of GFRα1 in minocycline group was higher( P 0. 05). Conclusion Minocycline can increase the relative expression of GFRα1 in rats with PD by inhibiting glial cells releasing cytokines and chemokines and other means,and thus play a biological role of dopaminergic neurons nutrition and protection.
Publication Year: 2014
Publication Date: 2014-01-01
Language: en
Type: article
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