Title: Effect of electroacupuncture on ERK1/2 signaling pathway of substantia nigra cells of rotenone-induced rats model with Parkinson's disease
Abstract: Objective To observe the effect of electroacupuncture( EA) on ERK1 /2 signaling pathway and inflammatory cytokines TNF-α in substantia nigra( SN) cells of rotenone-induced rats model with Parkinson's disease( PD),and explore the mechanism of EA on PD.Methods A total of 32 male SD rats were randomly and averagely divided into normal,sham-operation,model and EA groups. Model and EA groups were injected intradermally with rotenone( dissolved in DMSO and saline in certain percentage) on the nape of the neck for 14 d to establish PD model rats. Behavioral assessment was conducted after the establishment of PD rats model. EA group was applied to Fengfu and Taichong points for 20 min once daily for 14 d. Other groups were not given treatments. After the treatments,the rats were killed for sampling substantia nigra tissue to detect the number of TH,p-ERK1 /2 and TNF-α positive cells by inmmuno-histochemistry. Results Rats in each group performed differently in neurobehavior. Compared with normal and sham-operation groups,the expressions of TH positive cells were significantly reduced in model group( P0. 05),the expressions of p-ERK1 /2 and TNF-α positive cells were significantly increased in model group( P0. 05). Compared with that of model group,the expression of TH positive cells was significantly increased in EA group( P0. 05),the expressions of p-ERK1 /2 and TNF-α positive cells were significantly reduced in EA group( P0. 05). Conclusions EA therapy might reduce the expression of TNF-α in SN of PD rats by regulating the expression of p-ERK1 /2( MAPK pathway),which might delay the process of PD.
Publication Year: 2015
Publication Date: 2015-01-01
Language: en
Type: article
Access and Citation
AI Researcher Chatbot
Get quick answers to your questions about the article from our AI researcher chatbot